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Oxycodone is a drug that is used to control severe to moderate pains and as an anti-cough medication. Many people start taking Oxycodone as prescribed drug and eventually get addicted. When continuously exposed to Oxycodone, the body develops tolerance to the drug. The person requires high dosage each time to achieve same sense of relaxation, satisfaction, euphoria, and high. Addiction to oxycodone leads to seizures, dizziness, slowed breathing, and coma. Finding oxycodone drug presence using Urine drug testing is one the most convenient, easy, reliable, and cost effective methods.
Specimen Collection
The urine specimen that has to be tested for Oxycodone drug presence should be collected in a clean, dry, and unbreakable container with no leaks. The urine samples should be handled with care, as there is a risk of contracting infection from urine. A new container should be used every time for new urine specimen to avoid contamination of specimen. If testing has to be done later, the urine samples can be stored at 2-8 degree celsius temperature for 48 hrs or below -20 degree celsius for longer period. If any solid particles are seen in the urine sample, then it should be centrifuged, filtered, and allowed to settle down to get clear specimen for testing.
How to Conduct The Test
Laboratories use Immunoassay (IA) technique for initial screening of the urine sample for Oxycodone presence. The urine specimen and test device should be brought to room temperature before testing. The bottom part of the test strip should be inserted in the urine specimen and care should be taken that sample level is within the blue area of the test device. The test device should be kept inserted until reddish purple lines appear in the test results area. When such lines appear, remove the test strip, and lay it flat on non-absorbent surface. The test results can be read within just 3-5 minutes.
Interpretation of Results
The results can be read when reddish-purple lines appear in the Control region [C] and Test region [T]. The results are considered as negative when one reddish-purple line appears in Control region [C] and another reddish-purple line appears in Test region [T]. Even if the color of the two reddish-purple lines in Control region and Test region is light, results should be considered as negative. The negative result indicates that Oxycodone is present below cut off concentration of 100 ng/ml. The result should be interpreted as positive, when one line appears in Control region [C] and no line appears in Test region [T]. Positive result shows that Oxycodone is present in sample above the cut off concentration of 100 ng/ml. The result is considered as invalid if no line appears in Control region [C] and Test region [T] even after 10 minutes. In such case, testing should be performed with new strip. The result should also be considered as invalid if a line appears in test region [T] and no line appears in control region [C].
The positive result in urine drug testing indicates preliminary analytical results. More advanced techniques like Gas Chromatography/Mass Spectrometry can be used for confirmatory purposes. A positive result cannot show concentration level of drug and route of administration. Whereas, negative result indicates that drug is present below cut-off concentration level and not completely drug free urine.
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